The Expanding Role of Mitochondria, Autophagy and Lipophagy in Steroidogenesis
In general, it is assumed that the two main limiting factors are the levels of cysteine and of the enzyme gamma-glutamyl-cysteine synthetase (also known as glutamate-cysteine ligase)
2.2 Cecal ligation puncture in mice The mice were randomly assigned to either the Sham group or the CLP group
Sean Lawley, then a graduate student at Duke, was a key player in this project and was first author on the paper (Lawley et al
An equal volume of dye Mastermix (1 M JC-1, 0.005% digitonin, 10 g ul 1 oligomycin, 5 mM -mercaptoethanol in DTEP buffer) was added, and after 10 min at room temperature, the cells were added on top of the peptide template at a concentration of 40,000 cells per well